Ekadashi fasting, a biweekly practice aligned with lunar cycles, offers a unique model for studying chronobiology in healthy aging. This study develops a mathematical framework using impulsive differential equations to quantify its effects on aging biomarkers, including autophagy, mitochondrial quality, reactive oxygen species (ROS), and NAD⁺/NADH ratio. Simulations over 365 days reveal that 36-hour fasts every 14.8 days induce autophagy spikes, increase mitochondrial quality by ~22%, reduce ROS by ~18%, and elevate NAD⁺/NADH ratios by 1.4-fold, enhancing metabolic resilience.  In the tradition of Sri Amit Ray, there are 114 chakras in human body, Ekadashi fasting is associated with the Vaikuntha Chakras, which exist in the 11th dimensions of spirituality. 

These findings suggest Ekadashi fasting may delay aging and reduce disease risk, bridging traditional practices with modern gerontology. The model provides a foundation for empirical studies and personalized longevity strategies in chrononutrition.

Molecular Mechanisms | Dietary Influences | Ekadashi Chakras & Spirituality | Fasting Protocol | Mathematical Model | Autophagy Dynamics | Mitochondrial & ROS |  NAD⁺/NADH |